PTH intact ELISA

Enzyme immunoassay for the quantitative determination of Intact-PTH (Parathyroid Hormone) in human serum.

INTENDED USE: The PTH intact ELISA is intended for in vitro diagnostic use in the quantitative determination of Intact PTH (Parathyroid Hormone) in human serum. This assay is intended to be used to detect elevated or decreased PTH level in human serum, and to aid in the diagnosis of hyperparathyroidism and hypoparathyroidism for professional use.

GENERAL INFORMATION: PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, preproparathyroid hormone is converted to an intermediate, a 90-amino acid polypetide, proparathyroid hormone. With additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an 84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and midregion fragments. In subjects with renal insufficiency, C-terminal and midregion PTH assays typically give elevated PTH results, as reflected by impaired renal clearance.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 3 h, 30 min (RT/shaker)
- Standard Range: 14.2 - 1,094 pg/ml
- Sensitivity analytical: 0.2 pg/ml
- Final Sample Volume: 25 µl
- Sample Type:serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE