Protein C ELISA

Enzyme immunoassay for the quantitative determination of Protein C in citrated human plasma.

INTENDED USE:Protein C is a solid phase enzyme immunoassay for the quantitative determination of Protein C in citrated human plasma. The determination of Protein C aids in the risk estimation of thrombosis.

GENERAL INFORMATION:Protein C is a vitamin K-dependent inactive zymogen of a serine protease that is mainly synthesized by hepatocytes in the liver. It has a molecular weight of 62 kDa and is present at a concentration of 4 µg/ml in the plasma. Activated Protein C (aPC) is a key component of the Protein C anticoagulant system that is activated by the binding of thrombin to the endothelial transmembrane receptor thrombomodulin. The complex of thrombin and thrombomodulin activates Protein C and the activated Protein C in turn forms a complex with its cofactor Protein S that has a high affinity to phospholipid membranes. This is of physiological importance since aPC inactivates preferentially the membrane-bound coagulation factors Va and VIIIa. Additionally, activated Protein C possesses profibrinolytic activity by inhibiting plasmin activator inhibitor-1 (PAI-1). Protein C deficiency may be inherited or acquired and is associated with a variably increased risk of thrombosis. The prevalence of Protein C deficiency has been estimated to be up to one case per 300 in the general population. Nearly 50-80 % of individuals with inherited Protein C deficiency will experience a thrombotic event before the age of 30-45. Patients with a homozygous Protein C deficiency may suffer from neonatal purpura fulminans or massive venous thrombosis. Acquired Protein C deficiency is often associated with liver disease, surgery, oral anticoagulant therapy, antiphospholipid syndrome, etc. Protein C deficiency is classified in two states. Type I deficiency is a reduction in the level of Protein C. Type II deficiency is characterized by a reduced Protein C activity, with normal antigen level. To determine the type of defect, the laboratory diagnosis of Protein C may require both antigen levels and functional determination.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 30 min, 30 min (RT); 30 min (RT/dark)
- Standard Range: 12.5 - 150 %
- Sensitivity analytical: 6 %
- Final Sample Volume: 100 µl
- Sample Type: plasma
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE