PMN Elastase ELISA

Enzyme immunoassay for the quantitative measurement of PMN elastase in EDTA or citrated plasma, and seminal plasma.

INTENDED USE:The Demeditec PMN Elastase ELISA is an enzyme immunoassay for the quantitative determination of the complex of PMN Elastase and the α1-proteinase inhibitor (α1-PI) in human EDTA or citrated plasma and seminal plasma. The assay is intended for in-vitro diagnostic use by professional users only. All therapeutic conconsequences must take not only the test result but always also all clinical and laboratory diagnostic results into account. The laboratory values themselves must never be the sole reason for therapeutic consequences derived from them. Manual processing is validated. The usage of laboratory automates is the user’s sole responsibility. The kit is intended for single use only.

GENERAL INFORMATION:The human organism reacts with an inflammatory response to attacks of invading pathogens (microorganisms and viruses) or damaged tissue (after accidents or surgery). Polymorphonuclear (PMN) granulocytes play an important role as primary defense cells in this inflammatory reaction. Different bloodstream mediators (cytokines, leukotrienes, complement factors, bacterial endotoxins, clotting, and fibrinolysis factors) attract and stimulate these cells to phagocytose and destroy not naturally occurring agents. PMN granulocytes use proteinases to digest these agents and tissue debris. One of these proteinases is PMN Elastase, which is localized in the azurophilic granules of the PMN granulocytes. During phagocytosis of foreign substances, these enzymes are also partially excreted into the extracellular surrounding, where the activity of PMN Elastase is regulated by inhibitors (esp. the alpha1-proteinase inhibitor, alpha1-PI). An overwhelming release of PMN Elastase, however, can exceed the inhibitory potential of the alpha1-proteinase inhibitor. Thus, enzymatically active PMN Elastase, together with simultaneously produced oxidants (O2-radicals, H2O2, OH-radicals, etc.), can cause local tissue injury. Due to the bloodstream and lymphatic system, alpha1-PI is delivered subsequently and eventually able to form a complex with all excreted PMN Elastase. Therefore, the concentration of the PMN Elastase/alpha1-PI complex correlates with the released PMN Elastase and can be used as a measure for the activity of granulocytes during an inflammatory response. Primarily, determinations of PMN Elastase find its application as an adjunct in the diagnostics and observation of the course of trauma, shock and sepsis. Further indications are the areas of hemodialysis, infections by obstetrics, joint diseases, intestinal affection and pancreatitis. The determination of PMN Elastase in seminal plasma can be used as an adjunct in the diagnostics of male adnex affections.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 1 h (RT/shaking), 30 min (RT/dark)
- Standard Range: 0.16 - 10 ng/ml
- Sensitivity analytical: 0.1 ng/ml
- Final Sample Volume: 100 µl
- Sample Type: plasma (EDTA, citrate), seminal plasma
- Sample Pretreatment: dilution
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE