Plasma Renin Activity (PRA) ELISA

Enzyme immunoassay for the quantitative determination of Plasma Renin Activity (PRA) in human plasma.

INTENDED USE:For the quantitative measurement of Plasma Renin Activity (PRA) in human EDTA plasma by an ELISA (Enzyme-Linked Immunosorbent Assay). This kit is intended for professional use only and is for laboratory use only. For in vitro diagnostic use only. Intended to be used manually but may be adaptable to open automated analyzers. The user is responsible for validating the performance of this kit with any automated analyzers.

GENERAL INFORMATION: Plasma renin activity is an important diagnostic test for individualized therapy for resistant hypertension. It has been reported that measuring plasma renin activity significantly improved blood pressure control in patients with severe resistant hypertension. A randomized trial in the USA reported a reduction of medication required to achieve control using plasma renin activity to guide therapy. PRA is based on renin releasing angiotensin-I from angiotensinogen. Angiotensin-I is transformed to angiotensin-II largely in pulmonary circulation by the angiotensin-converting enzyme (ACE). Angiotensin-II raises blood pressure by direct arteriolar vasoconstriction, promoting sodium retention, and stimulating the secretion of aldosterone from the adrenal cortex. Aldosterone also exerts an effect to restore sodium balance and lift arterial pressure. Hence, the determination of plasma renin activity can aid in the diagnosis of primary hyperaldosteronism (5–13% of hypertensive cases) and assist in the therapy and management of other forms of hypertension.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 192
- Incubation Time / Conditions: 1 h, 30 min, 15 min (RT/shaker)
- Standard Range: 0.2 - 60 ng/ml
- Sensitivity analytical: LoD: 0.166 ng/ml
- Final Sample Volume: 50 µl
- Sample Type: plasma (EDTA)
- Sample Pretreatment: Generation Procedure
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE