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A photometer is an instrument that measures light absorbance at a specific wavelength, allowing the determination of the concentration of cells, proteins, or other substances in solution. It is widely used in microbiology and biotechnology laboratories as a practical and cost-effective alternative to a spectrophotometer. With an optical system typically set to 600 nm, it enables the application of various methods, including: (1) absorbance measurement (OD600) to estimate total cell concentration, (2) turbidity determination according to the McFarland (McF) scale, (3) protein quantification using the Bradford method, and (4) other methods that can be adapted or optimized for this wavelength.
Specifications of the various available models:- Measurement range: 0–3.0 Abs and 0–16.00 McF.- Measurement modes: Absorbance and McFarland (McF).- Wavelength: 600 nm.- Tube types: cuvettes, round tubes, Falcon tubes (12–16 mm).- USB connectivity.
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