Osteopontin human ELISA

Enzyme immunoassay for the quantitative determination of Human Osteopontin (Human OPN) in EDTA plasma, urine or cell culture supernatant.

INTENDED USE:This kit is to be used for the in-vitro quantitative determination of Human Osteopontin (Human OPN) in EDTA plasma, urine, or cell culture media. For research use only, not for use in diagnostic procedures.

GENERAL INFORMATION:Osteopontin (OPN) is a secreted glycoprotein that was originally isolated from bone. At present, it is known as a highly acidic calcium-binding glycosylated phosphoprotein secreted by many cell types, including osteoblasts, kidney tubule cells, macrophages, activated T cells, and vascular smooth muscle cells. Its molecular weights have been reported in the range of 66 kDa to 44 kDa depending on glycosylation and phosphorylation. One important feature of OPN is that it contains an Arg-Gly-Asp (RGD) amino acid sequence. This motif is present in fibronectin, vitronectin and a variety of other extra cellular proteins that bind members of the integrin family of cell surface receptors such as αvβ3. Another important of OPN is the presence of various molecular forms in vivo due to differential RNA splicing, glycosylation, phosphorylation, sulfation, and susceptibility to proteases. Both OPN and thrombin are likely to be localized together at the site of injury, inflammation, and angiogenesis and in tumor tissues. Osteopontin is susceptible to proteolytic fragmentation, and this process may have physiologic importance. Recently, it was shown that an amino terminal OPN fragment contains a cryptic binding site that can be recognized by α9β1 integrin. Furthermore, OPN contains multiple cell binding sites and interacts with various receptors; these interactions may have distinct functional.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 (4°C), 30 min (RT)
- Standard Range: 5 - 320 ng/ml
- Sensitivity analytical: 3.33 ng/ml
- Final Sample Volume: 100 µl
- Sample Type: urine, plasma (EDTA), cell culture supernatant
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO