N-ERC/Mesothelin human ELISA

Enzyme immunoassay for the quantitative determination Human N-ERC/ Mesothelin in EDTA plasma.

INTENDED USE:This assay kit is capable for the quantitative determination Human N-ERC/Mesothelin in EDTA plasma. For research use only, not for use in diagnostic procedures.

GENERAL INFORMATION:Erc has been identified as a gene showing stronger expression in cancer-affected renal tissue than in normal renal tissue in Eker rats (a rat model of renal cancer). The human homologue of the protein encoded by this gene is called MPF (megakaryocyte potentiating factor) or mesothelin. This protein is detected especially prominently in mesothelial cells, and its involvement has been suggested in the development of mesothelioma, making it a promising tumor marker. In humans, involvement of this protein has also been suggested in the development of pancreatic, ovarian and pulmonary cancers, etc. The protein is expressed as a GPI anchor-type membranous protein (about 71 kDa in molecular weight), which is digested by a furin-like protease to yield fragments about 31 kDa and 40 kDa in size. We have established a system for the assay of this protein, referring to the 31 kDa fragment as N-ERC/Mesothelin. This ELISA kit can measure N-ERC/Mesothelin in human plasma samples.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 (RT), 30 min (RT/dark)
- Standard Range: 0.07 - 4.4 ng/ml
- Sensitivity analytical: 0.017 ng/ml
- Final Sample Volume: 100 µl
- Sample Type: plasma (EDTA)
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO