Mouse Leptin Assay Kit

Background:The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman’s group at Rockefeller University originally identified the obesity gene that encodes for Leptin in 1994. Leptin is a 16 KDa secreted protein produced by the ob gene. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tends to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is also found that the protein acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. In addition, recent study shows Leptin acts as a potent inhibitor of bone formation through central nervous system.

Description:
This kit is a solid phase sandwich ELISA using 2 kinds of high specific antibodies. Tetra Methyl Benzidine (TMB) is used as coloring agent (Chromogen). The strength of coloring is in proportion to the quantities of Mouse Leptin.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 0,86 pg/mL