Leptin ELISA

Enzyme immunoassay for the quantitative determination of Leptin in human serum.

INTENDED USE: For the quantitative determination of Leptin in human serum by an enzyme immunoassay method.

GENERAL INFORMATION: Human Leptin is a 16 kDa, 146 amino acid residue, nonglycosylated polypeptide. Leptin is encoded by the OB gene. Its major source is the adipose tissue, and its circulating concentrations indirectly reflect body fat stores. Plasma or serum concentrations of leptin are increased in obese humans and strongly correlate with the degree of adiposity as expressed by percentage of body fat or body mass index. The recently discovered hormone leptin contributes to the regulation of energy balance by informing the brain of the amount of adipose tissue in the body. The brain may then make the appropriate adjustments in either energy intake or expenditure. Many areas of leptin physiology remain to be investigated. The roles of leptin in metabolism, insulin sensitivity, as a potential therapeutic modality for weight loss as well as involvement in endocrine function are active areas of research. While the future for leptin as a therapeutic agent is not clear, its involvement in many areas of physiology undoubtedly makes this a new hormone which requires extensive study in the future to understand its physiology.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 30min, 10-15 min (RT/shaker)
- Standard Range: 1 - 100 ng/ml
- Sensitivity analytical: LoD: 0.5 ng/ml
- Final Sample Volume: 20 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE