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Enzyme immunoassay for the determination of specific antibodies to bovine herpesvirus-1 (BHV-1) in bovine serum, plasma or milk using MAb-IBR-gB by blocking ELISA method.Description: The kit enables 192 tests, including controls. The whole kit may be used at one time, or sequentially for smaller batches of samples. Microtitre wells are coated with the purified and inactivated BHV-1 antigen. Total assay time according to sample incubation: over nigh at +2-8°C: results in a next day or 120 min: at 37°C: approximately 3,5 h The evaluation by the percentage of blocking is based on ratio of the difference between the average absorbance of the negative control serum and the absorbance of the sample to the average absorbance of the negative control serum expressed in percentage (%) Tested at the Veterinary Research Institute, Brno, Czech Republic and State Veterinary Institutes in the Czech Republic. Approved by the Institute for State Control of Veterinary Biologicals and Medicaments, Czech Republic. The kit is manufactured in accordance with Good Manufacturing Practice (GMP). Shelf life: 12 months. Application: Screening of IBR occurrence in a cattle population. Verification of clinical suspicion of disease appearance, Verification of virus circulation in herds. Obtaining the background information to formulate sanitation programmes for cattle herds or regions, control of current sanitation process in cattle herds. Evaluation of effects of the sanitation programme, ongoing screening in herds after sanitation. Control of transported and quarantined animals (export, import).