Human Leptin (highly sensitive) ELISA

Enzyme immunoassay for the quantitative determination of Human Leptin in serum, EDTA-plasma and cell culture supernatant.

INTENDED USE:This assay kit is capable for the quantitative determination of Human Leptin in serum, EDTA-plasma and supernatant of cell culture media.

GENERAL INFORMATION:The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman’s group at Rockefeller University originally identified the obesity gene that encodes for Leptin in 1994. Leptin is a 16 kDa secreted protein produced by the ob gene. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tend to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is also found that the protein acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. In addition, recent study shows Leptin acts as a potent inhibitor of bone formation through central nervous system. This ELISA kit has a high sensitivity for measuring Human Leptin.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 (4°C), 30 min (RT)
- Standard Range: 15.6 - 1,000 pg/ml
- Sensitivity analytical: 2 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (EDTA), cell culture supernatant
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO