Human APP βCTF Assay Kit

Background: Alzheimer’s disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of senile dementia. It is known that Amyloid β (Aβ; which is a major constituent of senile plaque) is cleaved from Amyloid Precursor Protein (APP; which exists in three main isoforms, APP695, APP751, and APP770) by β-secretase and subsequent γ-secretase. On the one hand, it has been also reported there is βCTF (β-secretase C-terminal fragment), fragments which are cleaved by β-secretase but not by γ-secretase. It is thought that measuring βCTF, in addition to Aβ, soluble APPα and APPβ, also has significance in research of the metabolic pathway of APP. This ELISA can measure human APP βCTF in samples such as cultured cell lysate.

Description:
This kit is a solid phase sandwich ELISA using 2 kinds of high specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantities of Human APP βCTF.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 0,02 pmol/L