Human Amyloid beta Oligomers (82E1-specific) ELISA

Enzyme immunoassay for the quantitative determination of human Aß molecules which bind to anti-human Aß (N) (82E1) mouse MoAb with two or more epitopes (oligomers, polymers with a protein etc.) in serum, EDTA-plasma and brain tissue extract.

INTENDED USE:This ELISA kit can detect human Aβ molecules which bind to anti-human Aβ (N) (82E1) mouse MoAb with two or more epitopes (oligomers, polymers with a protein etc.) in serum, EDTA-plasma and brain tissue extract.

GENERAL INFORMATION:Alzheimer's disease (AD) is a neurodegenerative disease which Amyloid beta (Aβ) peptide accumulates in a brain and the predominant symptom is a decline of cognitive functions. It has been generally thought that Aβ which condenses and become insoluble fiber (fibril) forms the senile plaque and this denatures neurons and develops the symptoms of the disease. (This is called the 'amyloid hypothesis'.) However, 'oligomer hypothesis' is becoming potent these days that the disease is developed by decline in cognitive function because of the soluble Aβ oligomer which is a middle object of a condensation process has strong synaptic injurious effects. It is reported that when the Aβ oligomer which is secreted from the cell or is extracted from AD patient's brain is injected into rat brain, it causes synaptic dysfunction and study memory loss. Moreover, it is also actually observed that the Aβ oligomers are increasing in brains of AD patients compared with healthy individuals.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 1 h (4°C), 30 min (RT)
- Standard Range: 18.98 - 1,215 pmol/l
- Sensitivity analytical: 4.41 pmol/l
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (EDTA), brain tissue extract
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO