Growth Hormone (hGH) ELISA

Enzyme immunoassay for the quantitative determination of Growth Hormone in human serum.

INTENDED USE:For the direct quantitative determination of Growth Hormone by an enzyme immunoassay in human serum.

GENERAL INFORMATION:Human growth hormone (hGH) is a polypeptide of 191 aminoacids secreted by the somatroph cells of the anterior pituitary. Growth hormone is principally a regulator of body growth and its metabolic effects are primarily anabolic. Some of its effects include promotion of protein conservation through its involvement in a wide range of protein synthesis mechanisms, enhancement of glucose transport and facilitation of glycogen storage. In addition, it induces the release of somatomedins (insulin-like growth factors), which further mediate the cascade of growth promoting actions. Measurement of hGH is primarily of interest in the diagnosis and treatment of various forms of decreased secretion of hGH. Hyposecretion of hGH in children results in growth retardation and hypersecretion leads to gigantism in children and acromegaly in adults. The secretion of hGH varies throughout the day under the influence of intricate neurogenic, metabolic and hormonal control. Due to the pulsatile nature of hGH release, it is often inaccurate to define a reference range and status based on single serum measurements. To diagnose disorders of hGH secretion more reliably, dynamic tests are used in which serum hGH levels are measured over a period following suppression or stimulation of hGH secretion.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 10-15 min (RT, shaker)
- Standard Range: 1 - 50 ng/ml
- Sensitivity analytical: 0.2 ng/ml
- Final Sample Volume: 25 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE