Glucagon ELISA

Enzyme immunoassay for the quantitative measurement of Glucagon in human EDTA plasma.

INTENDED USE:For the quantitative measurement of Glucagon in human EDTA plasma by an ELISA (Enzyme-Linked Immunosorbent Assay). For Research Use Only. Not for use in diagnostic procedures.

GENERAL INFORMATION: The Glucagon ELISA is a two-step capture or ‘sandwich’ type immunoassay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for glucagon is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of glucagon is conjugated to horse radish peroxidase (HRP conjugate). In the first incubation step, glucagon present in the specimen samples, calibrators and controls is bound to the antibody immobilized onto the microplate. Excess and unbound materials are removed by a washing step. In the second incubation step, HRP conjugate antibody (HRP conjugate) is added, which binds specifically to any immobilized glucagon, thus forming a sandwich complex. Unbound HRP conjugate is removed by a washing step. Next, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue coloured product that is directly proportional to the amount of glucagon present. The enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of glucagon in specimen samples and controls can be directly read.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 30 min, 15 min (RT)
- Standard Range: 11 - 352 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: plasma (EDTA)
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: RUO