GIP Total human ELISA

Enzyme immunoassay for the quantitative determination of human total GIP in EDTA-plasma and cell culture supernatant.

INTENDED USE: This assay kit is capable for the quantitative determination human total GIP in EDTA-plasma and cell culture supernatant.

GENERAL INFORMATION:ncretins are a group of gastrointestinal hormones that cause an increase in the amount of insulin released from the beta cells of the islets of Langerhans after eating and they also inhibit glucagon release from the alpha cells of the Islets of Langerhans. GIP, typical incretin like GLP-1, was isolated and sequenced from intestinal mucosa as “gastric inhibitory peptide” in 1970, and then it was renamed as “glucosedependent insulinotropic peptide”. It has been reported that GIP receptor is expressed in cells such as beta cell of pancreas, adipocyte or osteoblastic cell, and it plays essential roles in reserving of ingested nutrients within the body in each cell, and the control of GIP signal can lead to improvement of metabolic syndrome. GIP is rapidly inactivated to GIP (3-42) from active form of GIP (1-42) by DPPIV in blood. This ELISA kit detects both types of human GIP, active form (1-42) and inactivated form (3-42), and can measure total GIP in samples.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 1 h (4°C), 30 min (RT)
- Standard Range: 1.88 - 120 pmol/l
- Sensitivity analytical: 0.82 pmol/l
- Final Sample Volume: 100 µl
- Sample Type: plasma (EDTA), cell culture supernatant
- Sample Pretreatment: dilution
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO