Follicle Stimulating Hormone (hFSH) ELISA

Enzyme immunoassay for the quantitative determination of Follicle Stimulating Hormone in human serum.

INTENDED USE:For the direct quantitative determination of Follicle Stimulating Hormone by an enzyme immunoassay in human serum

GENERAL INFORMATION:Human follicle stimulating hormone (FSH) is a glycoprotein hormone produced by the anterior pituitary gland. There are three other glycoprotein hormones, namely Thyroid Stimulating Hormone, Luteinizing Hormone (both produced by anterior pituitary gland) and Human Chorionic Gonadotropin (produced by the placenta) which are structurally similar. Each hormone has an alpha and beta subunit. The α subunits of each hormone are similar while the β subunit is specifi c to each hormone. The α subunits contain 92 amino acids while the β subunits vary with each hormone. The β subunit of both FSH and LH contain 115 amino acids, TSH 110 amino acids, and hCG 147 amino acids.
The FSH and LH hormones function differently in females and males. It is to be noted that in women the growth and maturation of the ovarian follicle is dependent on FSH, while in men both LH and FSH act on the testes.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 30 min, 30 min, 15-20 min (RT/shaker)
- Standard Range: 5 - 100 IU/l
- Sensitivity analytical: 1 IU/l
- Final Sample Volume: 25 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE