FGF21 human ELISA

Enzyme immunoassay for the quantitative determination Human FGF21 in serum, EDTA plasma and cell culture supernatant.

INTENDED USE:This assay kit is capable for the quantitative determination Human FGF21 in serum, EDTA plasma and cell culture supernatant. Guideline of dilution for serum and EDTA plasma samples of normal human is around 2-8 fold. For research use only, not for use in diagnostic procedures.

GENERAL INFORMATION:FGF19 subfamily composed of FGF19, FGF21 and FGF23 binds the receptors, α/βKlotho and it is characterized as a factor group which has hormonal functions. The novel system consisted of α/βKlotho and FGF19 subfamily regulates integrally metabolism of minerals, lipids, energy and amino acids. This novel regulating system involves important homeostatic mechanism for biological body such as metabolism of bile acid and cholesterol in the liver (FGF19), energy and glycolipids metabolism (FGF21) and phosphate, calcium and vitamin D metabolism in kidney (FGF23) in fasting state. FGF21 is secreted in the liver and it is reported that it has a lipolytic action in fatty tissues. FGF21 binds a receptor consisted of FGFR and βKlotho. FGF21 reacts in emptiness and it accelerates lipid metabolism in liver, Triacylglycerol (TG) is used as lipid acid and Ketone bodies are produced by the metabolism. As a result, it is used as an energy source. In this process, FGF21 is released and acts as a hormone-like factor. In relation to the concentration of FGF21 in blood, it is measured and reported that the correlation with many diseases like gestational diabetes mellitus, mitochondrial dysfunction in HIV infection, friedreich ataxia, coronary artery disease, carotid atherosclerosis, non-alcoholic fatty liver disease, rheumatoid arthritis, diabetic nephropathy, type2 diabetes, dyslipidemia and, obesity and metabolic syndrome. This kit can assay Human blood FGF21 density.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: overnight, 30 (4°C), 30 min (RT)
- Standard Range: 31.3 - 2,000 pg/ml
- Sensitivity analytical: 29.4 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma, cell culture supernatant
- Sample Pretreatment: dilution
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO