E. coli O104:H4 Immunomagnetic Separation Beads

Immunomagnetic separation beads designed for the rapid and selective concentration of Escherichia coli serogroup O104:H4 to help with their confirmation on selective confirmation agars.

The IMS beads provided in the kit are coupled to antibodies against E. coli serotype O104:H4. The E. coli O104:H4 IMS beads should be used as part of the USDA-FSIS test protocol described in the USDA-FSIS Microbiology Laboratory Guidebook (MLG) Chapter 5 “Detection, Isolation and Identification of Top Seven Shiga-Toxin Producing Escherichia coli Strains (STEC) from Meat Products and Carcass and Environmental Sponges”.
The MLG utilizes sample enrichment in modified tryptic soy broth, followed by multiplexed Real Time-PCR assays targeting stx1, stx2 and eae genes and the wxz gene in the O-antigen gene clusters of the six serogroups, and then immunomagnetic separation (IMS) followed by plating onto Rainbow Agar (mRBA). Colonies are then tested for specific O antigens using latex agglutination and positive colonies are purified on Sheep Blood Agar (SBA) and confirmed using PCR and biochemical identification.

Related Kits:
- E. coli O26 IMS (GSD543000);
- E. coli O45 IMS (GSD543010);
- E. coli O103 IMS (GSD543020);
- E. coli O111 IMS (GSD543030);
- E. coli O121 IMS (GSD543040);
- E. coli O145 IMS (GSD543050);
- E. coli O104:H4 IMS (GSD543060);
- E. coli O157:H7 IMS (GSD543070).