Deoxynivalenol ELISA

Enzyme Immunoassay for the rapid quantitative determination of Deoxynivalenol in cereals and beer/gyle.

Background: Deoxynivalenol (DON, Vomitoxin) in addition to zearalenone, fumonisines and other trichothecenes belongs to the fusarium toxins. These toxins are already produced on the field in consequence of a contact of the cereals by fusarium species. Acute toxic dosages result in sickness and emesis. Deoxynivalenol is a gastrointestinal irritant and an inhibitor in protein synthesis. Farm animals react with a delay of growth and a depressed immune system resulting in a higher sensitivity for infections. Since June 2010 the US Food and Drug Association recommends maximum amounts of 1000 ppb for finished cereal products and 2000 - 10000 ppb for raw cereals depending on the intended use. Thus an monitoring of food and feed with respect to the concentration of deoxynivalenol is obligatory. The Deoxynivalenol ELISA represents a highly sensitive detection system and is particularly capable of the rapid quantification of deoxynivalenol contaminations in cereals and beer.

Description: The Deoxynivalenol ELISA quantitative test is based on the principle of the enzyme-linked immunosorbent assay. A microtiter plate is coated with antibodies raised against mouse immunglobulins. Deoxynivalenol containing samples or standards, a deoxynivalenol-peroxidase conjugate and an antibody directed against deoxynivalenol are given into the wells of the microtiter plate. The conjugate competes with the deoxynivalenol of samples/standards for the limited number of antibody sites. Simultaneously the anti-deoxynivalenol antibody is bound to the anti-mouse antibody coated on the microtiter plate. After 10 minutes incubation at room temperature the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 10 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of deoxynivalenol is indirectly proportional to the colour intensity of the test sample.

Product Features:
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- Deoxynivalenol Standards: 0, 0.2, 0.5, 1, 2, 5 ppm;
- ELISA reader at 450 nm;
- Standard range: 0.2 - 5 ppm;
- Sensitivity analytical: 0.08 ppm.