COX-2 human ELISA

Enzyme immunoassay for the quantitative determination of Human COX-2 in lysate of cultured cells.

INTENDED USE:This product is for research use only and is not intended for diagnostic use.

GENERAL INFORMATION:This kit is a solid phase sandwich ELISA (Enzyme-linked Immunosorbent Assay). As a primary antibody is coated on a plate, samples and standard are added into the wells for 1st reaction. After the reaction, HRP-conjugated secondary antibody is added into the wells for 2nd reaction. After washing away unbound the secondary antibody, Tetra Methyl Benzidine (TMB) is added to the wells and color develops.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 (4°C), 30 min (RT)
- Standard Range: 2.15 - 275 ng/ml
- Sensitivity analytical: 2 ng/ml
- Final Sample Volume: 100 µl
- Sample Type: lysate of cell pellet
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO