Cortisol ELISA

Enzyme immunoassay for the quantitative determination of Cortisol in human serum.

INTENDED USE:For the quantitative measurement of Cortisol in human serum by an ELISA (Enzyme-Linked Immunosorbent Assay). This kit is intended for professional use only and is for laboratory use only. For in vitro diagnostic use only. Intended to be used manually but may be adaptable to open automated analyzers. The user is responsible for validating the performance of this kit with any automated analyzers.

GENERAL INFORMATION:Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. Measurement of blood cortisol levels can be used as an indicator of adrenal function and the differential diagnosis of Addison’s and Cushing’s diseases as well as adrenal hyperplasia. Most circulating cortisol is bound to cortisol binding globulin or transcortin. Cortisol in blood shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 45, 15 min (RT)
- Standard Range: 0.4 - 40 µg/dl
- Sensitivity analytical: LoD: 0.298 µg/dl
- Final Sample Volume: 20 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE