Chorionic Gonadotropin (hCG) ELISA

Enzyme immunoassay for the quantitative determination of Chorionic Gonatropin in human serum.

INTENDED USE:For the direct quantitative determination of Chorionic Gonadotropin by enzyme immunoassay in human serum.

GENERAL INFORMATION:Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by the placenta. During normal pregnancy the level of this hormone in serum and urine gradually increases up to about the eighth week of pregnancy. hCG has two subunits, namely α and β. The α subunit is similar to the α subunit of the anterior pituitary gland glycoprotein hormones, namely, LH, TSH and FSH. However, the β subunits of these hormones are different. Therefore, this uniqueness distinguishes one hormone from the other, hence specifi city. In raising this monoclonal antibody, the immunogen used was βhCG, which helps to make the DBC assay system very specifi c and sensitive.
In normal pregnancy the increase in hCG starts at about the 5th day after conception and continues to rise until it reaches a maximum at about the eighth week. In some pathological conditions the level of hCG in serum and/or urine is increased. It is a well known fact that hCG is also a tumour marker which is very important in the diagnosis of choriocarcinoma. In the case of hydatiform mole, hCG is also elevated. In about 50% of patients with testicular teratomas the level of hCG is elevated. It is also relevant to note that hCG is a good indicator, in order to follow the response to treatment.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 10-15 min (RT/shaker)
- Standard Range: 2.5 - 500 IU/l
- Sensitivity analytical: 0.7 IU/l
- Final Sample Volume: 25 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE