cAMP Urine ELISA

Enzyme immunoassay for the quantitative measurement of cAMP in human urine.

INTENDED USE:For the quantitative measurement of cAMP (cyclic adenosine-3',5'-monophosphate) in human urine by an ELISA (Enzyme-Linked Immunosorbent Assay). For Research Use Only. Not for use in diagnostic procedures.

GENERAL INFORMATION: The cAMP Urine ELISA is a competitive immunoassay. Competition occurs between cAMP present in calibrators, controls, specimen samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-cAMP antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of cAMP present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of cAMP in specimen samples and controls can be directly read.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (RT), 20 min (RT)
- Standard Range: 3 - 300 pmol/ml
- Final Sample Volume: 100 µl
- Sample Type: urine
- Sample Pretreatment: dilution 1:100
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: RUO