Bio-Shield Histamine Extra Sensitive (ES)

It is an ELISA test kit for the quantitative determination of Histamine in Fresh, Frozen, Raw, and Canned Tuna

SUMMARY AND EXPLANATION:Histamine (HA) is a heterocyclic primary amine and is the by-product of the decarboxylation of the amino acid Lhistidine by the enzyme histidine decarboxylase. The latter is produced by certain bacteria that can be found in sea food. The most common fish species that tend to accommodate HA belong to the Scombridae family of fish (tuna, mahi mahi, marlin, bluefish, sardines, anchovy, bonito, herring and mackerel). After the fish are harvested the bacterial growth results in increasing histidine decarboxylase amounts which in turn induces HA production. High levels of HA may cause scombroid poisoning in humans which consists of various symptoms, such as rash, nausea, vomiting, diarrhea, hypotension, heart palpitations and muscle weakness. Most controlling government agencies worldwide have regulations regarding the amount of HA allowable in fish and fish products. HA levels in fish of good quality are usually below 10 ppm. HA values between 50 and 200 ppm are acceptable depending on the fish species and the country. Accurate and rapid determination of HA presence in commodities is of paramount importance.

KIT CHARACTERISTICS:

- Single-break strip plate or plates, with 48 wells (6 strips) or 96 wells (12 strips)
- Quantitative interpretation of results
- Limit of detection (LOD): 0.3 mg/kg
- Limit of quantification (LOQ): 0.5 mg/kg
- Quantification Range: 0.5-50 mg/kg
- Test time: 20 minutes
- High Specificity and Sensitivity
- Shelf Life: 12 months | Storage 2-8°C