APP770 human ELISA

Enzyme immunoassay for the quantitative determination human APP770 in EDTA-plasma, cerebrospinal fluid and cell culture supernatant.

INTENDED USE:This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantities of Human APP770.

GENERAL INFORMATION:Alzheimer's disease (AD) is a typical senile dementia and it is believed that accumulation of Amyloidβ (Aβ) in brain parenchyma is a major cause of the disease. Meanwhile, accumulation of Aβ has been also observed on cerebrovascular walls of nearly 90% of individuals suffering with AD. Aβ is known to be produced from Amyloid Precursor Protein (APP) by cleavage with two types of protease (β- and γ- secretase) and Aβ molecules which accumulate in brain parenchyma are mainly produced from APP expressed in neurons, APP695. In contrast to this, another different APP, APP770, was found to be expressed in cerebrovascular endothelial cells, and it has been newly reported that the Aβ produced from the APP770 can accumulate on cerebrovascular walls. This ELISA kit can measure human APP770 in EDTA-plasma, ceribrospinal fluid and cell culture supernatant.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: overnight, 30 min (4°C)
- Standard Range: 0.1 - 6.2 ng/ml
- Sensitivity analytical: 0.03 ng/ml
- Final Sample Volume: 100 µl
- Sample Type: plasma (EDTA), CSF, cell culture supernatant
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO