ApoB-100 human ELISA

Enzyme immunoassay for the quantitative determination of Human ApoB-100 in serum, EDTA-plasma and cell culture supernatant.

INTENDED USE:This IBL’s assay kit is capable for the quantitative determination of Human ApoB100 in serum, EDTA-plasma and cell culture media. For research use only. Not for use in diagnostic procedures.

GENERAL INFORMATION:Though the lipids (such as fat and cholesterol) are not soluble in water, apolipoproteins are carrier proteins that combine with lipids to form lipoprotein particles which are water-soluble and can be carried through water-based circulation (i.e., blood, lymph). Apolipoproteins are classified according to their forms and functions, and there are six major classes and several sub-classes. ApoB (ApolipoproteinB) is the primary apolipoprotein of LDL and is considered to reflect change of LDL level in blood. There are two types apolipoprotein in ApoB. One is ApoB-100 which correlates with cholesterol level well and is released as LDL and VLDL. Another is ApoB-48 which presents being incorporated into chylomicrons and correlates well with tryglyceride. Generally, the concentration of ApoB in blood has been measured as the total of ApoB-100 and ApoB-48. This kit can measure only ApoB-100 in plasma samples using ApoB-100 specific antibodies which don’t recognize ApoB-48. This highly sensitive sandwich ELISA can also measure ApoB-100 in cell culture media or various fractionated lipoprotein samples.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 (4°C), 30 min (RT)
- Standard Range: 0.13 - 8.4 µg/ml
- Sensitivity analytical: 0.03 µg/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (EDTA), cell culture supernatant
- Sample Pretreatment: plasma: 1:400 diluted
- Isotope / Substrate: TMB 450 nm
-Regulatory Status: RUO