Angiopoietin-like 4 human ELISA

Enzyme immunoassay for the quantitative determination human ANGPTL4 in serum and EDTA-plasma.

INTENDED USE:This assay kit is capable for the quantitative determination human ANGPTL4 in serum and EDTA-plasma.

GENERAL INFORMATION:Angiopoetin-like 4 (ANGPTL4) is a member of the angiopoietin/ANGPTL family. Human ANGPTL4 consists of 406 amino acids. Its protein structure is common to the angiopoietins, with a signal peptide directing secretion, an N-terminal coiled-coil domain (CCD), a linker, and a C-terminal fibrinogen-like domain (FLD). Full-length ANGPTL4 is secreted by liver and adipose tissues and cleaved to generate circulating CCD and FLD fragments. ANGPTL4 plays important roles in lipid and glucose metabolism. It inhibits lipoprotein lipase (LPL) activity by breaking the dimmer molecule, and elevates plasma TG level. Meanwhile, it is also implicated in breast cancer metastasis to lung via the regulation of vascular integrity. Both of two antibodies used in this ELISA kit recognize the N-terminal portion of human ANGPTL4 respectively.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 (4°C), 30 min (RT)
- Standard Range: 23.44 - 1,500 pg/ml
- Sensitivity analytical: 17 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (EDTA), cell culture supernatant
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO