Androstenedione free in Saliva ELISA

Competitive immunoenzymatic colorimetric method for the quantitative determination of Androstenedione concentration in saliva.

INTENDED USE:Competitive immunoenzymatic colorimetric method for the quantitative determination of Androstenedione concentration in saliva. Androstenedione Saliva ELISA is intended for laboratory use only.

GENERAL INFORMATION:Androstenedione (also known as Δ4-androstenedione) is a steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and estradiol. It is the common precursor of male and female sex hormones. Some androstenedione is also secreted into the plasma, and may be converted in peripheral tissues to testosterone and estrogens. Androstenedione has relatively weak androgenic activity, estimated at ~ 20% of testosterone. Secretion and production rates also exceed those of testosterone in women in whom significant extraadrenal conversion of androstenedione to testosterone occurs. In premenopausal women the adrenal glands and ovaries each produce about half of the total androstendione (about 3 mg/day). After menopause androstenedione production is about halved, primarily due to the reduction of steroid secreted by the ovary. Nevertheless, androstenedione is the principal steroid produced by the postmenopausal ovary. The high serum-saliva correlation for androstenedione suggests that individual differences in serum androstenedione levels may be accurately estimated using saliva as a non-invasive alternative specimen.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 15 min (RT)
- Standard Range: 20 - 1,000 pg/ml
- Sensitivity analytical: 5 pg/ml
- Final Sample Volume: 50 µl
- Sample Type: saliva
- Sample Pretreatment: freeze, thaw, centrifuge
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE