Amyloid beta (x-40) human ELISA

Enzyme immunoassay for the quantitative determination of human amyloid-beta 40 in EDTA-plasma, cerebrospinal fluids, serum, cell culture supernatant or extract of brain tissue.

INTENDED USE:This product is for research use only and is not intended for diagnostic use.

GENERAL INFORMATION:This kit is a solid phase sandwich ELISA (Enzyme-linked Immunosorbent Assay). As a primary antibody is coated on a plate, samples and standard are added into the wells for 1st reaction. After the reaction, HRP-conjugated secondary antibody is added into the wells for 2nd reaction. After washing away unbound the secondary antibody, Tetra Methyl Benzidine (TMB) is added to the wells and color develops.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: overnight, 1 h (4°C), 30 min (RT)
- Standard Range: 7.81 - 500 pg/ml
- Sensitivity analytical: 5 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma, CSF, cell culture supernatant, brain tissue extract
- Sample Pretreatment: dilution
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO