Amyloid beta (1-43) (FL) human ELISA

Enzyme immunoassay for the quantitative determination human Aß (1-43) in cerebrospinal fluid, brain extract of Tg mouse and cell culture supernatant.

INTENDED USE:This assay kit is capable for the quantitative determination Human Aβ (1-43) cerebrospinal fluid and cell culture supernatant.

GENERAL INFORMATION: The first case of Alzheimer's disease (AD) was defined and reported in 1907 by the German scientist, Dr. A. Alzheimer. His studies have shown that this is the main cause of dementia in the elderly. The plaques which appear in the brains of Alzheimer's disease patients are mostly constituted by the Amyloidβ protein (Aβ). Aβ is a peptide which consists of 40 or 42 (43) amino acids, and reports show that this is cleaved from β- and γ- secretase from the amyloid precursor protein. APP is a trans-membrane protein consisting of 695, 751, or 770 amino acids. Reports have shown many variants of Aβ exist and are clarified into the culture supernatant from the APP cDNA transfected mouse neuroblastoma cell. Many types of Aβ molecules are targeted in AD research. A process, Aβ40 is produced by cleaving from Aβ49 through Aβ46 and Aβ43, has been reported in tripeptide hypothesis, partly for that reason the interest in Aβ43 molecules has been growing. This kit can measure full-length (FL) molecules of Human Aβ (1-43).

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: overnight, 1 h (4°C), 30 min (RT)
- Standard Range: 2.34 - 150 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: brain extract of Tg mouse, cell culture supernatant, CSF
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO