Amyloid-beta (1-40) High Sensitive ELISA

Enzyme immunoassay for the quantitative determination of human amyloid-beta (1-40) in EDTA-plasma, cerebrospinal fluids, serum, cell culture supernatant or extract from brain tissue.

INTENDED USE:The Human Amyloidβ (1-40) (FL) Assay Kit is an ELISA kit for the quantitative determination of human Aβ (1-40) in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or extract from brain tissue.

GENERAL INFORMATION:The first case of Alzheimer's disease was defined and reported in 1907 by the German scientist, Dr. A. Alzheimer. His studies have shown that this is the main cause of dementia in the elderly. The plaques which appear in the brains of individuals who suffer AD are mostly constituted by the Amyloidβ protein (Aβ). Aβ is a peptide which consists of 40 or 42 (43) amino acids, and reports show that this is cleaved from β- and γ- secretase from amyloid precursor protein (APP). APP is a trans-membrane protein consisting of 695, 751, or 770 amino acids. Reports have shown many variants of Aβ exist and are clarified into the culture supernatant from the APP cDNA transfected mouse neuroblastoma cell. A highly specific monoclonal antibody (82E1) is used as a labeled antibody of this ELISA kit, and the antibody was developed to detect N-terminal of Aβ specifically and not to react with APP (ref. 4). This kit can measure only full-length molecules of Aβ (1-40) (“FL” means full-length).

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: overnight, 1 h (4°C), 30 min (RT)
- Standard Range: 1.56 - 100 pg/ml
- Sensitivity analytical: 1 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (EDTA), CSF, cell culture supernatant, extract from brain tissue
- Sample Pretreatment: dilution
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO