Amyloid beta (1-38) high sensitive human ELISA

Enzyme immunoassay for the quantitative determination human amyloid-ß (1-38) in cerebrospinal fluids and cell culture supernatant.

INTENDED USE:This assay kit is capable for the quantitative determination human amyloidβ (1-38) which held N terminal side completely in cerebrospinal fluids and cell culture supernatant.

GENERAL INFORMATION:The first case of Alzheimer's disease was defined and reported in 1907 by the German scientist, Dr. A. Alzheimer. His studies have shown that this is the main cause of dementia in the elderly. The plaques which appear in the brains of Alzheimer's disease patients are mostly constituted by the Amyloidβ protein (Aβ). Aβ is a peptide which consists of 40 or 42 (43) amino acids, and reports show that this is cleaved from β- and γ- secretase from the amyloid precursor protein. APP is a trans-membrane protein consisting of 695, 751, or 770 amino acids. Reports have shown many variants of Aβ exist and are clarified into the culture supernatant from the APP cDNA transfected mouse neuroblastoma cell. In 2001, it was found that the secretion of Aβ42 decreased by the effect of Nonsteroidal anti-inflammatory drugs (NSAIDs), and with this, the secretion of Aβ38 increased. NSAIDs is attracting attention as a γ- secretase modulation and then Aβ38 and Aβ42 have become important object molecules to be measured as indicators for the effect of NSAIDs. This ELISA kit can measure Human Aβ (1-38) which held N terminal side completely.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: overnight, 1 h (4°C), 30 min (RT)
- Standard Range: 9.38 - 600 pg/ml
- Sensitivity analytical: 1.28 pg/ml
- Final Sample Volume: 100 µl
- Sample Type: CSF, cell culture supernatant
- Sample Pretreatment: dilution
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO