25-Hydroxyvitamin D [25(OH)D] ELISA

Enzyme immunoassay for the quantitative determination of 25-hydroxyvitamin D in human serum and plasma.

INTENDED USE:For the quantitative determination of 25-hydroxyvitamin D [25(OH)D] in human serum and plasma by an enzyme immunoassay.

GENERAL INFORMATION:Vitamin D concentration in blood should be measured regularly to ensure that satisfactory physiological levels are maintained year round (see references). Vitamin D is assimilated from food sources (both vitamin D2 and vitamin D3) or produced in the skin by sun exposure (vitamin D3). The body stores both vitamin D2 and vitamin D3 mainly in the form of 25-hydroxyvitamin D2 or 25-hydroxyvitamin D3 respectively. Therefore, the best approach to assess the physiological levels of vitamin D is to analyze the total concentration of both hydroxylated forms [25(OH)D].

KIT CHARACTERISTICS:
-Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 30 min, 10 - 15 min (RT)
- Standard Range: 10 - 160 ng/ml
- Sensitivity analytical: LoD: 5.5 ng/ml
- Final Sample Volume: 25 µl
- Sample Type: serum, plasma (EDTA)
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE