17alpha-Hydroxyprogesterone (17alpha-OHP) ELISA

Enzyme immunoassay for the quantitative determination of 17alpha-hydroxyprogesterone in human serum.

INTENDED USE:For the direct quantitative determination of 17α-hydroxyprogesterone (17α-OHP) in human serum by an enzyme immunoassay.

GENERAL INFORMATION:The steroid 17α-hydroxyprogesterone is produced by the adrenal cortex and gonads. 17α-OHP has little progestational activity, but has intense clinical interest because it is the immediate precursor to 11-desoxycortisol, which is produced by the 21-hydroxylation of 17α-OHP. Measurement of 17α-OHP is, consequently, a useful indirect indicator of 21-hydroxlase activity. In congenital 21-hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17α-OHP is secreted in abundant excess. Measurement of 17α-OHP is therefore valuable in the initial diagnosis of CAH.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 15-20 min (RT)
- Standard Range: 0.15 - 20 ng/ml
- Sensitivity analytical: LoD: 0.051 ng/ml
- Final Sample Volume: 25 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE