17-OH-Progesterone free in Saliva ELISA

Background:The steroid 17-α-hydroxyprogesterone (17-α-OHP) is produced by both the adrenal cortex and gonads. Even though 17-α-OHP has relatively little progestational activity, it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Because Cpd-S is produced by 21-hydroxylation of 17-α-OHP, measurement of 17--OHP is a useful indirect indicator of 21hydroxylase activity. In congenital 21-hydroxylase deficiency, the most common variety of Congenital Adrenal Hyperplasia (CAH), 17-α-OHP is secreted in abundant excess. It is moderately elevated in the 11-β-hydroxylase deficiency as well. Measurement of 17-α-OHP is therefore valuable in the initial diagnosis of CAH.

Description:
The Salivary 17-OH Progesterone ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody (rabbit) directed towards a unique antigenic site of the 17-α-OHP molecule. Endogenous 17-α-OHP of a patient sample competes with a 17-α-OHP-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of 17-α-OHP in the sample. Having added of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of 17-α-OHP in the patient sample.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8 (break-apart) strips;
Standard: 0, 10, 50, 250, 500, 1000 pg/mL;
Microtiter plate reader at 450 nm;
Assay range: 3.6 - 1000 pg/mL;
Analytical Sensitivity: 2.5 pg/mL;
IVD