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Xpert One-Step Fast SYBR
Item code
GE40.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
GE40.0100: 100rxn
GE40.5100: 5x 100rxn
489
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One-Step qPCR: First-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. For intercalating dye applications
PRODUCT:
Using gene-specific primers (GSP), Xpert One-Step Fast SYBR allows for first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. Xpert One-Step Fast SYBR consists of a RTase mix, a Fast qPCR Mastermix (SYBR), and separated vial(s) of ROX.
The RTase mix comprises a genetically modified thermostable MMLV reverse transcriptase with improved synthesis efficiency, lacking any RNase H activity, and an advanced RNase inhibitor to impede RNA degradation.
The Fast qPCR Mastermix (SYBR) consists of the combination of a highly efficient enzyme with a novel low inhibitory technology. The intercalating dye used in this mastermix causes little to no inhibition of the PCR reaction thus allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products. Xpert One-Step Fast SYBR can be used to quantify virtually any RNA target, whether using mRNA, viral RNA or total RNA as template, including extremely low-copy number targets, with minimal effort and optimization.
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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