ViroReal® Kit Rabies Virus

Detection of RNA of the N gene of RABV

Background: Rabies is caused by a neurotropic virus of the genus Lyssavirus of the family Rhabdoviridae, and is transmissible to all mammals. As it is transmissible to humans by inoculation or inhalation of infectious virus, all suspected infected material must be handled under the appropriate safety conditions specified by the World Health Organisation (WHO). Previous known lyssavirus genotypes were recently reclassified, and the Lyssavirus genus currently consists of 14 lyssavirus species (Aravan virus, Australian bat lyssavirus (ABLV), Bokeloh bat lyssavirus (BBLV), Duvenhage virus (DUVV), European bat lyssavirus 1 (EBLV-1), European bat lyssavirus 2 (EBLV-2), Ikoma lyssavirus (IKOV), Irkut virus, Khujand virus, Lagos bat virus (LBV), Mokola virus (MOKV), Rabies virus (RABV), Shimoni bat virus (SHIBV) and the West Caucasian bat virus (WCBV). All lyssaviruses cause the severe neurotropic disease known as rabies. RABV (genotype 1, RABV-GT-1) is detected worldwide and is the prototype lyssavirus, the remaining species are known as rabies-related lyssaviruses. Human infections are mainly caused by RABV, DUVV, EBLV-1, EBLV-2 and ABLV.

Description: ViroReal® Kit Rabies Virus uses one-step reverse transcription RT real-time PCR for the detection of the nucleoprotein (N) gene of rabies virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: N gene of Rabies Virus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX