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ViroReal® Kit Encephalomyocarditis virus (EMCV)
Item code
DVEV01011
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Ingenetix
Variants
DVEV01011: 100 reactions - FAM, VIC/HEX
DVEV01013: 100 reactions - FAM, Cy5
DVEV01051: 50 reactions - FAM, VIC/HEX
DVEV01053: 50 reactions - FAM, Cy5
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Detection of RNA of the 3´UTR of encephalomyocarditis virus
Background:
Encephalomyocarditis virus (EMCV) is one of three virus species in the genus Cardiovirus in the family Picornaviridae and is a single stranded RNA (ssRNA) virus that causes encephalomyocarditis and reproductive disease in swine and zoologic mammals. Infected piglets suffer from encephalitis, myocarditis and sudden death. Reproductive problems include poor conception rates, embryo resorption, mummification, stillbirths, abortions and neonatal death. The disease affects the nervous, reproductive, respiratory and circulatory system of pigs. Fatal myocarditis due to EMC virus infection has been seen in primates, even-and odd-toed ungulates, elephants, carnivores and rodents. The disease can be found worldwide. The most important mode of virus transmission seems to be rodent-to-pig transmission, and rodents act as the major reservoir of the disease. Rats and other rodents probably infect pigs directly or through diseased carcasses and contaminated feeds or water supplies.
Description:
ViroReal® Kit EMCV uses one-step reverse transcription RT real-time PCR for the detection of the 3´UTR RNA of EMCV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.
Product Features:
- Amplification and detection: 3´UTR of EMCV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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