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ViroReal® Kit caprine arthritis encephalitis virus (CAEV)
Item code
DVEV03411
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Ingenetix
Variants
DVEV03411: 100 reactions - FAM, VIC/HEX
DVEV03413: 100 reactions - FAM, Cy5
DVEV03451: 50 reactions - FAM, VIC/HEX
DVEV03453: 50 reactions - FAM, Cy5
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Detection of RNA of the gag polyprotein gene of caprine arthritis encephalitis virus (CAEV)
Background:
The caprine arthritis - encephalitis virus (CAEV) of goats is an enveloped, single-stranded RNA small ruminant lentivirus (SRLV) in the family Retroviridae. There are several, genetically distinct isolates of the virus that differ in virulence. The CAE-virus is closely related to the Maedi-Visna-virus (MVV) of sheep. Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. Transmission of the CAEV is through ingestion of virus-infected goat milk but also through virus-infected secretion of affected animals and droplet infection. Clinical symptoms such as severe arthritis of the carpal joint are seen in approximately 20 % of infected goats. Subclinical or clinical interstitial pneumonia, mastitis with lower milk production and chronic wasting have also been attributed to infection with the virus.
Description:
ViroReal® Kit CAEV uses one-step reverse transcription RT real-time PCR for the detection of the gag polyprotein gene of Caprine Arthritis and Encephalitis Virus (CAEV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.
Product Features:
- Amplification and detection: gag gene of CAEV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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qPCR / Real Time PCR/ PCR
Others
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