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ViroReal® Kit bovine viral diarrhea (BVD) Virus
Item code
DVEV00613
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Ingenetix
Variants
DVEV00613: 100 reactions - FAM, Cy5
DVEV00653: 50 reactions - FAM, Cy5
DVEV00651: 50 reactions - FAM, VIC/HEX
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Detection of RNA of the 5´UTR of bovine viral diarrhea virus (BVDV-1, BVDV-2 and BVDV-3) and of some strains of the classical swine fever virus (CSFV) and border disease virus of sheep (BDV) (FLI approved)
Background:
The genus Pestivirus is composed of four important pathogens: bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2), classical swine fever virus (CSFV), and border disease virus of sheep (BDV). Recently a new putative pestivirus species called “HoBi-like,” “BVDV-3,” or “atypical pestivirus,” was identified. The BVD virus is a single strain, positive-sense RNA virus found throughout the world and causes bovine viral diarrhoea (BVD). BVD is a disease of cattle, which causes various clinical syndromes including diarrhea, mucosal disease, reproductive dysfunctions and/or hemorrhagic syndrome. Prenatal infection by BVD results in cattles permanently infected (BVD-PI). Such animals carry the virus throughout their lifetime and constantly secrete large numbers of viral particles.
Description:
ViroReal® Kit BVD Virus uses one-step reverse transcription RT real-time PCR for the detection of 5´UTR of BVDV-1, BVDV-2 and BVDV-3. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.
Product Features:
- Amplification and detection: 5´UTR of BVD Virus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: Cy5/ VIC/HEX
- Kit approved by Friedrich-Loeffler-Institute (Nr. FLI-B661)
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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