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TAE Buffer
Item code
GB11.0110
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
GB11.0110: 1L 10X
GB11.0510: 5L 10X
434
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Wide range of solutions, commonly used for molecular biology applications
PRODUCT:
TAE Buffer (10X) is an aqueous solutions of 400mM Tris, 200mM acetic acid, and 10mM EDTA, prepared with ultrapure water, and 0.2 μm filtered. TAE is the most common buffer used for agarose gel electrophoresis in the analysis of DNA samples and is recommended for high resolution of large DNA fragments (>3kb) or supercoiled DNA, however, it can also be used for non-denaturating RNA agarose electrophoresis.
Usage: The working concentration is either 1X or 0,5X. Prepare 1L TAE Buffer (1X) by mixing 100ml of the 10x concentrated buffer with 900ml of ddH2O. A 1X TAE buffer consists of 40mM Tris?acetate, 1mM EDTA at pH 8.3±0.1. TAE should be used both for the preparation of the agarose gel as well as the running buffer. Note that compared with other electrophoresis buffers, such as TBE or SGTB, TAE has a low ionic strength and low buffering capacity and may become exhausted during extended electrophoresis (>4h). The running buffer should therefore be replaced frequently.
Storage: TAE Buffer (10X) should be stored at room temperature and is stable for up to one and a half years.
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Filters
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ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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