T2 Toxin ELISA

Enzyme Immunoassay for the rapid quantitative determination of T2 Toxin in food.

Background: T-2 Toxin in addition to deoxynivalenol, zearalenone, the fumonisines and other trichothecenes belongs to the fusarium toxins. These toxins are already produced on the field in consequence of a contact of the cereals by fusarium species. Acute toxic dosages can result in gastroenteritis, damage of bone marrow up to necrosis of skin and respiratory passages. T-2 toxin has a high stability against temperature and can therefore also be detected in bakery products. In Russia the legislator set limit values for T-2 Toxin in food between 50 and 100 ppb. The introduction of limit values in the European union is discussed since many years. Thus an observation of food and feed with respect to the concentration of T-2 Toxin is increasingly obligatory. T2 Toxin ELISA represents a highly sensitive detection system and is particularly capable of the rapid quantification of T-2 Toxin contaminations in cereals, beer, milk and meat.

Description: The T2 Toxin ELISA quantitative test is based on the principle of the enzyme-linked immunosorbent assay. An antibody binding protein is coated on the surface of a microtiter plate. The standards and samples respectively are pipetted together with a T-2 Toxin-peroxidase conjugate and a rabbit-anti-T-2 Toxin antibody into the appropriate wells. The conjugate competes with the T-2 Toxin of samples/standards for the limited number of antibody sites. Simultaneously the anti-T-2 Toxin antibody is bound to the antibody-binding protein coated on the microtiter plate. After 10 min incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 10 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of T-2 Toxin is indirectly proportional to the colour intensity of the test sample.

Product Features:
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- T-2 Toxin Standards: 0, 17.5, 87.5, 350, 875, 1750 ppb;
- ELISA reader at 450 nm;
- Standard range: 17.5 - 1750 ppb;
- Sensitivity analytical: 13 ppb.