qPCR / Real Time PCR/ PCR
100 or 10 reactions

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Please choose the number of reactions

BACKGROUND: Legionnaires’ disease is the most severe form of infection, caused by Legionella strains, which includes pneumonia, and the fatality rate can approach 50% in immunocompromised patients. Outbreaks of Legionnaires’ disease have been worldwide traced to a wide variety of environmental water sources such as cooling towers, hot tubs, showerheads, whirlpools and spas, and public fountains. Over the last few years, the reported incidence of legionellosis has steadily increased. Commonly used method for environmental surveillance of Legionella spp. is the standard culture technique, but it has several limitations as it requires selective media and prolonged incubation periods. Recently, rapid and sensitive alternative methods have been found to be attractive alternatives to the conventional culture method for the detection of slow-growing and fastidious bacteria such as Legionella spp. These methods are PCR based methods, in particular using real time PCR and specific fluorescent probes, that allow the rapid detection of Legionella spp. in water samples. PRODUCT DESCRIPTION: SUPREME REAL TIME DETECTION KIT Legionella spp. uses real-time PCR for the detection of Legionella spp. in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal control (IC).To minimize PCR cross-contamination, the Kit contains dUTP and uracil-N glycosylase (UDG). PRODUCT FEATURES: -Amplification and detection of Legionella spp. -Real-time PCR with rapid hot-start Taq DNA polymerase -Internal Control to exclude false-negative results -Optimized to handle PCR inhibitors, containing UDG enzyme -Higher stability, enzymes in seperate tubes -PCR- platforms: runs on all established standard real-time PCR- platforms -Harmonized thermal profiles to run with other SUPREME BIOPREMIER kits simultaneously -Channel Target: FAM, Channel IC: ROX -Available formats: Kits with 100 or 10 reactions

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