qPCR / Real Time PCR/ PCR
100 or 10 reactions

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BACKGROUND: Escherichia coli (E. coli) is a bacterium that is commonly found in the gut of humans and warm-blooded animals and most of the strains are harmless. Some strains however, such as enterohemorrhagic E. coli (EHEC) O157:H7 can cause serious foodborne outbreaks that causes diarrhoea, fever and vomiting in humans.
E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms.
The reservoir of this pathogen appears to be mainly cattle, in addition to others such as ruminants, mammals and birds E. coli O157:H7 is transmitted to humans primarily through consumption of contaminated foods, such as raw or undercooked ground meat products and raw milk. Faecal contamination of water and other foods, as well as cross-contamination during food preparation (with beef and other meat products, contaminated surfaces and kitchen utensils), will also lead to infection.
The need for rapid, accurate, and sensitive methods for the detection of these E. coli strains is a major food safety issue. Since conventional microbiological methods for their detection and identification are time-consuming. Today, there are approved methods for PCR based detection of pathogenic genes of Escherichia coli unique DNA sequences, in particular using real time PCR and specific fluorescent probes.

PRODUCT DESCRIPTION: SUPREME REAL TIME DETECTION E. coli O157:H7 / O157 uses real-time PCR based on the use of TaqMan technology for the detection of pathogenic E. coli O157 and the simultaneous detection of the serotype O157:H7 DNA, providing a simple, reliable and rapid result for the detection of E. coli O157:H7 / O157 infection.
The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the target as well as a positive control and an internal control (IC). To minimize PCR cross-contamination,the Kit contains dUTP and uracil-N glycosylase (UDG)

-Amplification and detection: E. coli O157 and the serotype H7
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-Optimized to handle PCR inhibitors, containing UDG enzyme
-Higher stability, enzymes in separate tubes
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other SUPREME BIOPREMIER kits simultaneously
-Channel Targets: FAM and HEX/VIC, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions