English (United Kingdom)
Português (Portugal)
Home
products
Services
articles
About Us
Contact Us
My account
Wish List
View Cart (0)
English (United Kingdom)
Português (Portugal)
Home
products
Services
articles
About Us
Contact Us
_CAT_SearchNF
select
start
GO
COOKIES - This website uses its own and third party cookies to help provide you with the best experience we can. By clicking “I Accept" or scrolling down this page you accept cookies.
I Accept
Read Full Policy
My Account
Wish List
Cart
Menu
CAT_MenuNF
Menu
Equipments (59)
Agitators - Incubators - Vortex (9)
Autoclave Dry Heat Sterilizer (2)
Automatic Pipettors - Liquid dispensers (1)
Balances (2)
Baths - Heating thermoblock (3)
Centrifuges (4)
Electrodes pH - Conductivity - Oxygen (3)
Electrophoresis (7)
Equipments ELISA (4)
Equipments Lateral flow (3)
Equipments Real Time PCR (1)
Freezers - Refrigerators (2)
Homogenizer - Food homogenizer (3)
Microbiological incubators - Chambers (5)
Micropipettes - Electronic Pipettes (3)
Microscopes (2)
PCR - Laminar Flow Chambers (3)
Spectrophotometers (2)
Ultrasounds - Sonicators (0)
Water Purifier (0)
Food (242)
Beverages quality (3)
Food adulteration (28)
Food allergens (67)
Food safety (45)
Genetically modified organisms (GMO) (6)
Mycotoxins (40)
Plant pathogens (27)
Water quality (19)
Other food tests (7)
Human (126)
Bacteria (51)
Covid-19 tests (16)
Fungi (8)
Hormones (0)
Parasites (7)
Virus (42)
Other human tests (0)
General laboratory solutions (173)
Antibiotics, enzymes and buffers (23)
Cloning and cell research (27)
Culture media (27)
DNA/RNA amplification and analysis (42)
DNA/RNA extraction and purification (37)
Plasticware (17)
Veterinary (378)
Bacteria (171)
Fungi (9)
Hormones (5)
Parasites (29)
Virus (158)
Other microrganisms (6)
Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
_CAT_SearchNF_mobile
Search
select
start
{1}
##LOC[OK]##
{1}
##LOC[OK]##
##LOC[Cancel]##
{1}
##LOC[OK]##
##LOC[Cancel]##
Shrimp Alkaline Phosphatase (1U/uL)
Item code
GE015.0001
Technology
Others
Manufacturer
GRiSP
Variants
1mL (1.000U)
380
Add To Wish List
Request quote
Heat-labile multipurpose alkaline phosphatase that catalyzes the dephosphorylation of DNA, RNA and nucleotides. This recombinant enzyme replaces native SAP because it is much more stable at room temperature and is available at higher concentrations.
PRODUCT:
Recombinant Shrimp Alkaline Phosphatase (rSAP) is a heat-labile multipurpose alkaline phosphatase that catalyzes the dephosphorylation of DNA, RNA, and nucleotides. This recombinant enzyme replaces native SAP because it is much more stable at room temperature and is available at higher concentrations. For activity, rSAP requires magnesium (>1mM) and is tolerant to a wide variety of buffer conditions (salt, pH, etc), and thus can be added directly to many molecular biology buffers, including PCR mixtures and most restriction enzyme buffers. rSAP is completely inactivated by incubation at 65ºC for 5 minutes.
Application: Dephosphorylation of dNTPs from PCR reaction mixtures prior to DNA sequencing (typically in a PCR Clean-up protocol1 in combination with the use of Exonuclease I (Exo I)). Dephosphorylation of DNA prior to end-labelling (using T4 Polynucleotide Kinase). Dephosphorylation of vectors (plasmids) during cloning, in order to prevent recircularization during ligation reaction (using T4 DNA Ligase).
Menu
Equipments (59)
Agitators - Incubators - Vortex (9)
Autoclave Dry Heat Sterilizer (2)
Automatic Pipettors - Liquid dispensers (1)
Balances (2)
Baths - Heating thermoblock (3)
Centrifuges (4)
Electrodes pH - Conductivity - Oxygen (3)
Electrophoresis (7)
Equipments ELISA (4)
Equipments Lateral flow (3)
Equipments Real Time PCR (1)
Freezers - Refrigerators (2)
Homogenizer - Food homogenizer (3)
Microbiological incubators - Chambers (5)
Micropipettes - Electronic Pipettes (3)
Microscopes (2)
PCR - Laminar Flow Chambers (3)
Spectrophotometers (2)
Food (242)
Beverages quality (3)
Food adulteration (28)
Food allergens (67)
Food safety (45)
Genetically modified organisms (GMO) (6)
Mycotoxins (40)
Plant pathogens (27)
Water quality (19)
Other food tests (7)
Human (126)
Bacteria (51)
Covid-19 tests (16)
Fungi (8)
Parasites (7)
Virus (42)
General laboratory solutions (173)
Antibiotics, enzymes and buffers (23)
Cloning and cell research (27)
Culture media (27)
DNA/RNA amplification and analysis (42)
DNA/RNA extraction and purification (37)
Plasticware (17)
Veterinary (378)
Bacteria (171)
Fungi (9)
Hormones (5)
Parasites (29)
Virus (158)
Other microrganisms (6)
_CAT_SearchNF_filtros
Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
_CAT_DashBoard
{1}
##LOC[OK]##
{1}
##LOC[OK]##
##LOC[Cancel]##
{1}
##LOC[OK]##
##LOC[Cancel]##
Featured Products
Real Time Pcr Detection Test Kit Food Safety Salmonella Spp.
28
View Options
Newsletter
×