Enzyme immunoassay for the quantitative determination of Sesame in food.
Background: Sesame belongs to the family of Pedaliaceae. With about 16-32% the fraction of proteins in sesame seed is very high. Some of these proteins, like the albumins Ses i 1 and Ses i 2 or the globulin Ses i 3 are known for being allergenic. Because of its wide- spread application possibilities, sesame is used in many food preparations. For sesame-allergic persons hidden sesame allergens in food are a critical problem. Already very low amounts of sesame can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, sesame-allergic persons must strictly avoid the consumption of sesame containing food. Cross-contamination, mostly in consequence of the production process, is often noticed. This explains why in many cases the existence of sesame residues in food cannot be excluded. For this reason sensitive detection systems for sesame residues in foodstuffs are required.
Sesame ELISA represents a highly sensitive detection system for sesame and is particularly capable of the quantification of residues in bakery products, soups, sausage, sauces and ice cream.
Description: The sesame quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against sesame proteins is bound on the surface of a microtiter plate. Sesame containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugated second antibody directed against sesame proteins is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of sesame is directly proportional to the colour intensity of the test sample.
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- Sesame Standards: 0, 2, 5, 15, 30 ppm;
- ELISA reader at 450 nm;
- Standard range: 2 - 30 ppm;
- Sensitivity analytical: 0.2 ppm.