English (United Kingdom)
Português (Portugal)
Home
products
Services
articles
About Us
Contact Us
My account
Wish List
View Cart (0)
English (United Kingdom)
Português (Portugal)
Home
products
Services
articles
About Us
Contact Us
_CAT_SearchNF
select
start
GO
COOKIES - This website uses its own and third party cookies to help provide you with the best experience we can. By clicking “I Accept" or scrolling down this page you accept cookies.
I Accept
Read Full Policy
My Account
Wish List
Cart
Menu
CAT_MenuNF
Menu
Equipments (63)
Agitators - Incubators - Vortex (9)
Autoclave Dry Heat Sterilizer (2)
Automatic Pipettors - Liquid dispensers (1)
Balances (2)
Baths - Heating thermoblock (3)
Centrifuges (4)
Electrodes pH - Conductivity - Oxygen (3)
Electrophoresis (7)
Equipments ELISA (4)
Equipments Lateral flow (3)
Equipments Real Time PCR (1)
Freezers - Refrigerators (2)
Homogenizer - Food homogenizer (3)
Microbiological incubators - Chambers (5)
Micropipettes - Electronic Pipettes (3)
Microscopes (2)
PCR - Laminar Flow Chambers (3)
Spectrophotometers (2)
Ultrasounds - Sonicators (2)
Water Purifier (2)
Food (254)
Beverages quality (3)
Food adulteration (30)
Food allergens (75)
Food safety (47)
Genetically modified organisms (GMO) (6)
Mycotoxins (40)
Plant pathogens (27)
Water quality (19)
Other food tests (7)
Human (122)
Bacteria (51)
Covid-19 tests (12)
Fungi (8)
Hormones (0)
Parasites (7)
Virus (42)
Other human tests (0)
General laboratory solutions (177)
Antibiotics, enzymes and buffers (23)
Cloning and cell research (27)
Culture media (27)
DNA/RNA amplification and analysis (42)
DNA/RNA extraction and purification (41)
Plasticware (17)
Veterinary (378)
Bacteria (171)
Fungi (9)
Hormones (5)
Parasites (29)
Virus (158)
Other microrganisms (6)
Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
_CAT_SearchNF_mobile
Search
select
start
{1}
##LOC[OK]##
{1}
##LOC[OK]##
##LOC[Cancel]##
{1}
##LOC[OK]##
##LOC[Cancel]##
Rt - Real Time Pcr Detection Test Kit Supreme Hlvd (Hop Latent Viroid)
Item code
BIOPSFS-0064
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Biopremier
Variants
BIOPSFS-0064: 500 reactions - FAM, Cy5
BIOPSFS-sp0064: 100 reactions - FAM, Cy5
Instructions
(pdf)
Instructions for use
671
Select Options
Please indicate the product variant you want to quote:
Add To Wish List
Request quote
Detection of Hop latent viroid (HLVd) in Cannabis or other plant samples. The BIOPREMIER SUPREME RT-PCR Detection Kit HLVd combines a reverse transcriptase with a multiplex qPCR assay to simultaneously detect HLVd and an Internal Control. The kit is suitable for use in real time instruments with FAM and Cy5 detection channels.
BACKGROUND:
Hop latent viroid (HLVd) is a single-stranded, circular infectious RNA that is dependent on its host plant’s metabolism for replication. HLVd infection occurs worldwide in hops (Humulus lupulus) but it can also infect the cannabis plant. The HLVd-infected plants don’t show obvious symptoms of infection but instead grow shorter with smaller leaves, which reduce the production of certain cultivars. HLVd is usually spread via infected tools or through cloning (cuttings taken from an infected mother).
Because symptoms of these viruses are not always obvious, regularly screening by qPCR is the key to isolating the infected plants from their growing area to prevent the spread of the virus throughout the productive field.
PRODUCT DESCRIPTION:
SUPREME REAL TIME DETECTION KIT HLVd uses Reverse Transcriptase (RT) real-time PCR for the detection of HLVd in a simple, reliable, and rapid procedure. The assay combines a reverse transcriptase with a multiplex qPCR assay based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence of each target. Our carefully designed primers and probe ensure the highest sensitivity and specificity. The kit consists of an assay mix for detecting the targets, a positive control, and internal control (IC).To minimize PCR cross-contamination, the Kit contains dUTP and uracil-N glycosylase (UDG).
PRODUCT FEATURES:
-Amplification and detection of HLVd
-Transcription ensured by the enzyme Reverse transcriptase
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-Optimized to handle PCR inhibitors, containing UDG enzyme
-Higher stability, enzymes in seperate tubes
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Channel Target: FAM
-Channel IC: Cy5
-Available formats: Kits with 100 or 500 reactions
{1}
##LOC[OK]##
{1}
##LOC[OK]##
##LOC[Cancel]##
{1}
##LOC[OK]##
##LOC[Cancel]##
Related Products
Rt - Real Time Pcr Detection Test Kit Supreme Hlvd / Lcv / Cancv
670
View Options
Menu
Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
_CAT_SearchNF_filtros
Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
Newsletter
×