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REAL TIME PCR DETECTION TEST KIT Water Legionella pneumophila

BIOPFS-0015
qPCR / Real Time PCR/ PCR
Biopremier
BIOPFS-0015: 100 reactions - FAM, ROX
BIOPFS-sp0015: 10 reactions - FAM, ROX
(pdf) Instructions for use

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REAL TIME PCR DETECTION TEST KIT Water Legionella pneumophila

Detection of L. pneumophila in water samples. Water samples shall be concentrated according to ISO 11731. The kit is suitable for use in real time instruments with FAM and ROX detection channels.

BACKGROUND: Legionella pneumophila is a causative agent of Legionnaires’ disease. It was first recognized in 1977 following an epidemic of acute pneumonia among veterans of the American Legion in Philadelphia and led to the discovery of a new bacterial species and genus. The vast majority of Legionnaires’ disease cases are due to L. pneumophila, being serogroup 1 responsible for up to 80% of reported cases. Legionnaires’ disease is the most severe form of infection, which includes pneumonia, and the fatality rate can approach 50% in immunocompromised patients. Outbreaks of Legionnaires’ disease have been worldwide traced to a wide variety of environmental water sources such as cooling towers, hot tubs, showerheads, whirlpools and spas, and public fountains. Over the last few years, the reported incidence of legionellosis has steadily increased. Commonly used method for environmental surveillance of Legionella is the standard culture technique, but it has several limitations as it requires selective media and prolonged incubation periods. Recently, rapid and sensitive alternative methods have been found to be attractive alternatives to the conventional culture method for the detection of slow-growing and fastidious bacteria such as Legionella. These methods are PCR based methods, in particular using real time PCR and specific fluorescent probes, that allow the rapid detection of Legionella pneumophila in water samples.

PRODUCT DESCRIPTION: REAL TIME DETECTION KIT Legionella pneumophila uses real-time PCR for the detection of Legionella pneumophila in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal control (IC).

PRODUCT FEATURES:
-Amplification and detection of Legionella pneumophila
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Pathogen: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions

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