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Real Time Pcr Detection Test Kit Food Safety Salmonella Spp.
Item code
BIOPFS-0001
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Biopremier
Variants
BIOPFS-0001: 100 reactions - FAM, ROX
BIOPFS-sp0001: 10 reactions - FAM, ROX
Instructions
(pdf)
Instructions for use
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Detection of Salmonella spp. in food products, animal feedstuff and environmental samples. Pre-enrichment broth according to ISO 6579 (recommended). For use in real time PCR instruments with FAM and ROX channels.
BACKGROUND:
Salmonella spp. continues to be a major foodborne pathogen for animals and humans. In many countries, it is the leading cause of food-borne infections and outbreaks and due to the worldwide trade of food and feeding stuff, the risk of microbial infections for consumers increases. Culture-based methods used for Salmonella spp. detection often results in 4 - 6 days of isolation procedures.
In vitro amplification of DNA by the polymerase chain reaction (PCR) has become a powerful alternative in microbiological diagnostics due to its rapidity and accuracy. Today, there are approved methods for PCR based detection of Salmonella spp. unique DNA sequences, in particular using real time PCR and specific fluorescent probes.
PRODUCT DESCRIPTION:
REAL TIME DETECTION KIT Salmonella spp. uses real-time PCR for the detection of Salmonella spp. in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and internal control (IC).
PRODUCT FEATURES:
-Amplification and detection of Salmonella spp.
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Pathogen: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions
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Equipments (63)
Agitators - Incubators - Vortex (9)
Autoclave Dry Heat Sterilizer (2)
Automatic Pipettors - Liquid dispensers (1)
Balances (2)
Baths - Heating thermoblock (3)
Centrifuges (4)
Electrodes pH - Conductivity - Oxygen (3)
Electrophoresis (7)
Equipments ELISA (4)
Equipments Lateral flow (3)
Equipments Real Time PCR (1)
Freezers - Refrigerators (2)
Homogenizer - Food homogenizer (3)
Microbiological incubators - Chambers (5)
Micropipettes - Electronic Pipettes (3)
Microscopes (2)
PCR - Laminar Flow Chambers (3)
Spectrophotometers (2)
Ultrasounds - Sonicators (2)
Water Purifier (2)
Food (242)
Beverages quality (3)
Food adulteration (28)
Food allergens (67)
Food safety (45)
Genetically modified organisms (GMO) (6)
Mycotoxins (40)
Plant pathogens (27)
Water quality (19)
Other food tests (7)
Human (126)
Bacteria (51)
Covid-19 tests (16)
Fungi (8)
Parasites (7)
Virus (42)
General laboratory solutions (176)
Antibiotics, enzymes and buffers (23)
Cloning and cell research (27)
Culture media (27)
DNA/RNA amplification and analysis (42)
DNA/RNA extraction and purification (40)
Plasticware (17)
Veterinary (378)
Bacteria (171)
Fungi (9)
Hormones (5)
Parasites (29)
Virus (158)
Other microrganisms (6)
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